Hotspot

rpoB-trnC-GCA

LSC · 25860-27154 · 1295 bp

Back to Hemipilia

Pi 0.0221
MarkerSeek score 48.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1295
variable_sites
43
pi
0.0221
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG CCTTACCACTTGGCCATGC 50.0 57.9 58.2 58.8 1422-1442 bp 1.0 67.5
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG GCCTTACCACTTGGCCATG 50.0 57.9 58.2 58.8 1423-1443 bp 1.0 67.5
rpoB-trnC-GCA_p3 AAATTCTTCCGTCAAGCCCT CCTTACCACTTGGCCATGC 45.0 57.9 57.4 58.8 1423-1443 bp 1.0 64.1
rpoB-trnC-GCA_p4 AAATTCTTCCGTCAAGCCCT GCCTTACCACTTGGCCATG 45.0 57.9 57.4 58.8 1424-1444 bp 1.0 64.1
rpoB-trnC-GCA_p5 CTTCCGTCAAGCCCTGATTA CCTTACCACTTGGCCATGC 50.0 57.9 57.3 58.8 1418-1438 bp 1.0 63.3