Hotspot

atpH-atpI

LSC · 12804-13513 · 710 bp

Back to Hemipilia

Pi 0.0225

MarkerSeek score 64.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 24
pi: 0.0225
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCAGCGGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	726-768 bp	1.0	86.1
atpH-atpI_p2	CCCGCGGAAACAAAAGAATC	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1081-1122 bp	1.0	85.9
atpH-atpI_p3	GCCACGACCAGTCCATAAAT	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	906-948 bp	1.0	85.7
atpH-atpI_p4	CCCGCGGAAACAAAAGAATC	CTGCGCCCACGAATGATATA	50.0	50.0	58.0	57.9	1417-1458 bp	1.0	85.1
atpH-atpI_p5	CCCGCGGAAACAAAAGAATC	TTAGCTTTACCCACGTCAGC	50.0	50.0	58.0	57.9	1382-1423 bp	1.0	85.0