Hotspot

trnS-GCU-trnG-UCC

LSC · 7824-8350 · 527 bp

Back to Hemipilia

Pi 0.0354

MarkerSeek score 57.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 28
pi: 0.0354
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	AACGGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	611-625 bp	1.0	69.2
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	AACGGAAAGACCCCTTAACT	55.0	45.0	58.5	56.4	606-620 bp	1.0	68.4
trnS-GCU-trnG-UCC_p3	GCTTTAGTCCACTCAGCCAT	AAACGGAAAGACCCCTTAACT	50.0	42.9	57.6	57.1	612-626 bp	1.0	67.3
trnS-GCU-trnG-UCC_p4	AGTCCACTCAGCCATCTCTC	AAACGGAAAGACCCCTTAACT	55.0	42.9	58.5	57.1	607-621 bp	1.0	66.5
trnS-GCU-trnG-UCC_p5	TAGTCCACTCAGCCATCTCT	AACGGAAAGACCCCTTAACT	50.0	45.0	57.2	56.4	607-621 bp	1.0	65.8