Hotspot

rps16-trnQ-UUG

LSC · 6054-6478 · 425 bp

Back to Hemipilia

Pi 0.0206

MarkerSeek score 62.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 12
pi: 0.0206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	441-493 bp	1.0	83.8
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	457-509 bp	1.0	83.7
rps16-trnQ-UUG_p3	GCACGTTGCTTTTTACCACA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	57.8	57.8	445-497 bp	1.0	82.7
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	461-513 bp	1.0	82.6
rps16-trnQ-UUG_p5	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	445-497 bp	1.0	82.4