Hotspot

trnK-UUU-rps16

LSC · 4280-4868 · 589 bp

Back to Hemipilia

Pi 0.0374
MarkerSeek score 60.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
589
variable_sites
33
pi
0.0374
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GGATCAGTCGCGGTATTCTC TCGATCCCGAAGAGAAGGAA 55.0 50.0 57.9 57.9 888-926 bp 1.0 83.9
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 804-842 bp 1.0 83.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 803-841 bp 1.0 83.4
trnK-UUU-rps16_p4 GAGACAGAGGGCCCAATCTA TCGATCCCGAAGAGAAGGAA 55.0 50.0 58.2 57.9 1497-1538 bp 1.0 82.6
trnK-UUU-rps16_p5 GGATCAGTCGCGGTATTCTC CCAAGGATTTCACAAAGCGC 55.0 50.0 57.9 58.3 1333-1334 bp 0.666667 63.9