Hotspot

matK-trnK-UUU

LSC · 3212-4242 · 1031 bp

Back to Hemipilia

Pi 0.031
MarkerSeek score 70.30
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
1031
variable_sites
48
pi
0.031
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCCTTGTAATCCTGTCATTTACA ATCCGGGTTGCTAACTCAAC 34.8 50.0 55.3 57.9 1115-1168 bp 1.0 45.2
matK-trnK-UUU_p2 TCCTTGTAATCCTGTCATTTACA AACGGTAGAGTACTCGGCTT 34.8 50.0 55.3 58.2 1098-1151 bp 1.0 45.2
matK-trnK-UUU_p3 TCCTTGTAATCCTGTCATTTACA ACGGTAGAGTACTCGGCTTT 34.8 50.0 55.3 58.2 1097-1150 bp 1.0 45.2
matK-trnK-UUU_p4 TCCTTGTAATCCTGTCATTTACA GATCCGGGTTGCTAACTCAA 34.8 50.0 55.3 57.6 1116-1169 bp 1.0 45.1
matK-trnK-UUU_p5 TCCTTGTAATCCTGTCATTTACA TTGATCCGGGTTGCTAACTC 34.8 50.0 55.3 57.6 1118-1171 bp 1.0 45.1