Hotspot

ycf1

SSC · 122674-127770 · 5097 bp

Back to Hemionitis

Pi 0.183

MarkerSeek score 72.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5097
variable_sites: 930
pi: 0.183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCCGCATCTTCCCTTTCAT	ACAGAAGTTTCAAGCCCTGG	50.0	50.0	58.0	58.0	795 bp	0.5	59.5
ycf1_p2	TACATTCCCGGGTCTGAGTT	ACAGAAGTTTCAAGCCCTGG	50.0	50.0	58.1	58.0	1277 bp	0.5	59.5
ycf1_p3	GTCCGCATCTTCCCTTTCAT	TTCAGACTTGAACCAACCCG	50.0	50.0	58.0	58.0	859 bp	0.5	59.3
ycf1_p4	CCACCGAAAGATGTAGTCCG	ACAGAAGTTTCAAGCCCTGG	55.0	50.0	58.1	58.0	810 bp	0.5	59.3
ycf1_p5	TACATTCCCGGGTCTGAGTT	TTCAGACTTGAACCAACCCG	50.0	50.0	58.1	58.0	1341 bp	0.5	59.3