Hotspot

ndhB

LSC · 1-1109 · 1109 bp

Back to Hemionitis

Pi 0.204

MarkerSeek score 79.20

Primer available yes

Label ndhB

Feature Metadata

label_name: ndhB
feature_type: gene
region: LSC
length_bp: 1109
variable_sites: 226
pi: 0.204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	GTGTCTCTACGTTAGGAATCGT	TATCGATGAGTGGAGCGAGT	45.5	50.0	57.4	58.0	604 bp	0.5	38.8
ndhB_p2	GTGTCTCTACGTTAGGAATCGT	GGTATCCATGGCAGTCAGTG	45.5	55.0	57.4	58.0	951 bp	0.5	38.8
ndhB_p3	GTGTCTCTACGTTAGGAATCGT	TGGAGCAGCTTTCAAACTGT	45.5	45.0	57.4	57.9	447 bp	0.5	38.6
ndhB_p4	GTGTCTCTACGTTAGGAATCGT	GGATGGTTCTTAGCTGTGCA	45.5	50.0	57.4	57.9	736 bp	0.5	38.2
ndhB_p5	GTGTCTCTACGTTAGGAATCGT	TTCCATCGCTTCTGAACGTT	45.5	45.0	57.4	57.8	903 bp	0.5	37.8