Hotspot

rpl32-trnP-GGG

SSC · 112284-112911 · 628 bp

Back to Hemionitis

Pi 0.339

MarkerSeek score 82.20

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: SSC
length_bp: 628
variable_sites: 191
pi: 0.339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	CAAGGGCCGAATCTTACACA	CTCTGGCACTGCTTCCTAAG	50.0	55.0	57.9	58.0	1467-1513 bp	1.0	91.0
rpl32-trnP-GGG_p2	CAAGGGCCGAATCTTACACA	CTGGCACTGCTTCCTAAGAG	50.0	55.0	57.9	58.0	1465-1511 bp	1.0	91.0
rpl32-trnP-GGG_p3	GCACTTCTGGATCGAAGAGG	CTCTGGCACTGCTTCCTAAG	55.0	55.0	58.1	58.0	1133 bp	0.5	59.3
rpl32-trnP-GGG_p4	GCACTTCTGGATCGAAGAGG	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.1	58.0	1131 bp	0.5	59.3
rpl32-trnP-GGG_p5	GCACTTCTGGATCGAAGAGG	AGAGCAGCGTGTCTACCTAT	55.0	50.0	58.1	57.9	1115 bp	0.5	59.0