Hotspot

petA-psbJ

LSC · 59065-60070 · 1006 bp

Back to Hemionitis

Pi 0.251

MarkerSeek score 83.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1006
variable_sites: 237
pi: 0.251
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCGAGGAGTCATCTGAGGG	CCGAATAAACAGAGCGTGGA	55.0	50.0	58.0	58.0	1627 bp	0.5	59.9
petA-psbJ_p2	ATCGAGGAGTCATCTGAGGG	TCCGAATAAACAGAGCGTGG	55.0	50.0	58.0	58.0	1628 bp	0.5	59.9
petA-psbJ_p3	ATCGAGGAGTCATCTGAGGG	CAGAGAGCCGACAAGAAGTC	55.0	55.0	58.0	58.0	1875 bp	0.5	59.9
petA-psbJ_p4	ATCGAGGAGTCATCTGAGGG	TCTTCCTCGGGGTCCATATC	55.0	55.0	58.0	58.0	1708 bp	0.5	59.9
petA-psbJ_p5	TGGCCAGGGAGAAGTAGAAA	CCGAATAAACAGAGCGTGGA	50.0	50.0	58.0	58.0	1499 bp	0.5	59.7