Hotspot

ndhC-trnV-UAC

LSC · 47771-48186 · 416 bp

Back to Hemionitis

Pi 0.2
MarkerSeek score 80.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
416
variable_sites
80
pi
0.2
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CATATTGGTGAAACAGAAACA GGTTCGAATCCGTATAGCCC 33.3 55.0 52.3 57.9 460-505 bp 1.0 50.7
ndhC-trnV-UAC_p2 TCATATTGGTGAAACAGAAACA GGTTCGAATCCGTATAGCCC 31.8 55.0 53.9 57.9 461-506 bp 1.0 50.6
ndhC-trnV-UAC_p3 TCATATTGGTGAAACAGAAACA CGGTTCGAATCCGTATAGCC 31.8 55.0 53.9 58.3 462-507 bp 1.0 50.6
ndhC-trnV-UAC_p4 TCATATTGGTGAAACAGAAACAT GGTTCGAATCCGTATAGCCC 30.4 55.0 54.5 57.9 461-506 bp 1.0 50.6
ndhC-trnV-UAC_p5 TCATATTGGTGAAACAGAAACAT CGGTTCGAATCCGTATAGCC 30.4 55.0 54.5 58.3 462-507 bp 1.0 50.6