Hotspot

psbD-trnT-GGU

LSC · 33693-34933 · 1241 bp

Back to Hemionitis

Pi 0.227

MarkerSeek score 86.40

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1241
variable_sites: 261
pi: 0.227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TCTTTCGGTTCTTTGGAAGA	CGTAAGTCGTCGGTTCAAGT	40.0	50.0	54.5	58.0	1244-1315 bp	1.0	63.9
psbD-trnT-GGU_p2	AGATTTTCCAATGGCAATTGTCA	CGTAAGTCGTCGGTTCAAGT	34.8	50.0	57.5	58.0	1227-1298 bp	1.0	63.8
psbD-trnT-GGU_p3	TCTTTCGGTTCTTTGGAAGA	GCCCTTTTAACTCAGCGGTA	40.0	50.0	54.5	57.9	1283-1354 bp	1.0	63.4
psbD-trnT-GGU_p4	AGATTTTCCAATGGCAATTGTCA	GCCCTTTTAACTCAGCGGTA	34.8	50.0	57.5	57.9	1266-1337 bp	1.0	63.3
psbD-trnT-GGU_p5	TTTTCCAATGGCAATTGTCA	CGTAAGTCGTCGGTTCAAGT	35.0	50.0	54.2	58.0	1224-1295 bp	1.0	61.5