Hotspot

psbM-petN

LSC · 28130-29062 · 933 bp

Back to Hemionitis

Pi 0.254

MarkerSeek score 83.10

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 933
variable_sites: 221
pi: 0.254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCTCTACATTCAAACGGCCG	ATGTAACTCGACACGTCACG	50.0	50.0	58.0	58.0	1351 bp	0.5	59.6
psbM-petN_p2	ATCTCGGGGGATTAGGGAAG	ATGTAACTCGACACGTCACG	55.0	50.0	58.0	58.0	1804 bp	0.5	59.4
psbM-petN_p3	TTATTGCCTACTTGGTCGGC	ATGTAACTCGACACGTCACG	50.0	50.0	58.0	58.0	2026 bp	0.5	59.4
psbM-petN_p4	TCTCTACATTCAAACGGCCG	GACAAGGTTTTCCGGGGATT	50.0	50.0	58.0	58.1	1437 bp	0.5	59.3
psbM-petN_p5	TCTCTACATTCAAACGGCCG	GTTTGGGGAAGAAGTGGGTT	50.0	50.0	58.0	57.9	994 bp	0.5	59.3