Hotspot

rpoB-trnD-GUC

LSC · 26216-27061 · 846 bp

Back to Hemionitis

Pi 0.266

MarkerSeek score 82.10

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 218
pi: 0.266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	TAAATCTCCCTCCCCGATCC	CTTCCCTAATCCCCCGAGAT	55.0	55.0	58.0	58.0	1895 bp	0.5	59.5
rpoB-trnD-GUC_p2	TAAATCTCCCTCCCCGATCC	GAATCCAGCTCGACCCAATT	55.0	50.0	58.0	58.0	1507 bp	0.5	59.4
rpoB-trnD-GUC_p3	GGCATCTCTGTCTTCGACTG	CTTCCCTAATCCCCCGAGAT	55.0	55.0	58.1	58.0	1627 bp	0.5	59.1
rpoB-trnD-GUC_p4	TAAATCTCCCTCCCCGATCC	AATAACTCCGACCCAACGTG	55.0	50.0	58.0	57.9	1411 bp	0.5	59.1
rpoB-trnD-GUC_p5	GGCATCTCTGTCTTCGACTG	GAATCCAGCTCGACCCAATT	55.0	50.0	58.1	58.0	1239 bp	0.5	59.0