Hotspot

atpH-atpI

LSC · 13270-14023 · 754 bp

Back to Hemionitis

Pi 0.221

MarkerSeek score 82.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 161
pi: 0.221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGAGTTATCCACTTCGGCAA	ACTTCTCGGGTTGTTATCGC	50.0	50.0	58.0	58.0	1910 bp	0.5	59.9
atpH-atpI_p2	TCCCTCCTTGAAGGTTTTGC	ACTTCTCGGGTTGTTATCGC	50.0	50.0	58.0	58.0	2019 bp	0.5	59.9
atpH-atpI_p3	CGAGTTATCCACTTCGGCAA	ATTTTGGCTGACGAGTTGGT	50.0	45.0	58.0	58.0	1451 bp	0.5	59.8
atpH-atpI_p4	TCCCTCCTTGAAGGTTTTGC	ATTTTGGCTGACGAGTTGGT	50.0	45.0	58.0	58.0	1560 bp	0.5	59.8
atpH-atpI_p5	CGAGTTATCCACTTCGGCAA	CAGCCAGAAATTTGCAGTCG	50.0	50.0	58.0	58.0	1864 bp	0.5	59.7