Hotspot

matK-chlB

LSC · 2810-4586 · 1777 bp

Back to Hemionitis

Pi 0.237

MarkerSeek score 81.30

Primer available yes

Label matK-chlB

Feature Metadata

label_name: matK-chlB
feature_type: igs
region: LSC
length_bp: 1777
variable_sites: 408
pi: 0.237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-chlB_p1	GTCAGCGGAAGTGAATCGAT	CCGGATTCGTAAGGGGAAAG	50.0	55.0	58.1	58.1	2582 bp	0.5	59.0
matK-chlB_p2	GTCAGCGGAAGTGAATCGAT	ACGTATGCGGCTAAGGAAAG	50.0	50.0	58.1	58.1	2499 bp	0.5	58.9
matK-chlB_p3	GTCAGCGGAAGTGAATCGAT	CCTGAAGCTGAGTCGGAATC	50.0	55.0	58.1	58.1	2613 bp	0.5	58.9
matK-chlB_p4	GTCAGCGGAAGTGAATCGAT	GCCCTCTGCCATATTTGGAA	50.0	50.0	58.1	57.9	2890 bp	0.5	58.8
matK-chlB_p5	GTCAGCGGAAGTGAATCGAT	CCTTTCCTGGGTTACGAAGG	50.0	55.0	58.1	57.9	2775 bp	0.5	58.5