Hotspot

rps16

LSC · 4952-6075 · 1124 bp

Back to Hemiboea

Pi 0.00996

MarkerSeek score 54.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1124
variable_sites: 56
pi: 0.00996
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GATACGAACCGAGCAGGAAG	AAACATGCTGCTTATTGGCG	55.0	45.0	58.2	57.7	1720 bp	0.0625	39.0
rps16_p2	TAGATACGAACCGAGCAGGA	AAACATGCTGCTTATTGGCG	50.0	45.0	57.7	57.7	1722 bp	0.0625	37.5
rps16_p3	GATACGAACCGAGCAGGAAG	AACATGCTGCTTATTGGCGA	55.0	45.0	58.2	58.5	1719 bp	0.0625	36.9
rps16_p4	GATACGAACCGAGCAGGAAG	ACATGCTGCTTATTGGCGAA	55.0	45.0	58.2	58.5	1718 bp	0.0625	36.9
rps16_p5	AGATACGAACCGAGCAGGAA	AAACATGCTGCTTATTGGCG	50.0	45.0	58.5	57.7	1721 bp	0.0625	36.0