Hotspot

ndhD-psaC

SSC · 116928-117028 · 101 bp

Back to Hemiboea

Pi 0.0433

MarkerSeek score 66.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 101
variable_sites: 18
pi: 0.0433
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTTGCCCACTGTACATTGCT	GATACCCTGGGACGGATGTA	45.0	55.0	58.0	58.0	652-658 bp	1.0	85.3
ndhD-psaC_p2	TTTGCCCACTGTACATTGCT	GACCGAAGACTGTGTTGGTT	45.0	50.0	58.0	58.1	604-610 bp	1.0	85.2
ndhD-psaC_p3	TTTGCCCACTGTACATTGCT	CCGACCGATTTCTTGAGTGT	45.0	50.0	58.0	57.9	561-567 bp	1.0	85.2
ndhD-psaC_p4	TTTGCCCACTGTACATTGCT	AAATGATACCCTGGGACGGA	45.0	50.0	58.0	58.1	656-662 bp	1.0	84.4
ndhD-psaC_p5	TTTGCCCACTGTACATTGCT	AATGATACCCTGGGACGGAT	45.0	50.0	58.0	57.9	655-661 bp	1.0	84.3