Hotspot

psbT-psbN

LSC · 75094-75158 · 65 bp

Back to Hemiboea

Pi 0.0464

MarkerSeek score 69.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 8
pi: 0.0464
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	47.6	50.0	57.6	57.8	117-118 bp	1.0	73.0
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	47.6	50.0	57.6	57.8	118-119 bp	1.0	73.0
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	47.6	55.0	57.6	58.3	120-121 bp	1.0	72.1
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	ATTCGAGGAACATGGGGACT	47.6	50.0	57.6	58.4	119-120 bp	1.0	71.5
psbT-psbN_p5	TCGAGAACCACCTAAAGTTCC	TCGAGGAACATGGGGACTAA	47.6	50.0	57.3	57.8	118-119 bp	1.0	70.7