Hotspot

rpl20-rps12

LSC · 69773-70535 · 763 bp

Back to Hemiboea

Pi 0.00733

MarkerSeek score 62.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 30
pi: 0.00733
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	864-932 bp	1.0	78.8
rpl20-rps12_p2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	919-987 bp	1.0	78.0
rpl20-rps12_p3	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	918-986 bp	1.0	78.0
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	827-895 bp	1.0	77.4
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	881-949 bp	1.0	76.6