Hotspot

trnT-GGU-psbD

LSC · 32001-33322 · 1322 bp

Back to Hemiboea

Pi 0.00864

MarkerSeek score 66.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1322
variable_sites: 57
pi: 0.00864
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1475-1511 bp	1.0	82.3
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1478-1514 bp	1.0	82.1
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1465-1501 bp	1.0	81.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1468-1504 bp	1.0	81.0
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CCCTTCGTAACCAGTCATCC	55.0	55.0	58.1	57.7	1449-1485 bp	1.0	80.7