Hotspot

rps2-rpoC2

LSC · 16535-16734 · 200 bp

Back to Hemiboea

Pi 0.0279

MarkerSeek score 75.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 200
variable_sites: 22
pi: 0.0279
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCTTCCAGCATCTCTTCCAA	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.9	57.8	358-376 bp	1.0	85.8
rps2-rpoC2_p2	AATGAACTCCTGCTTCCAGC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.2	57.8	369-387 bp	1.0	85.2
rps2-rpoC2_p3	CCAGCATCTCTTCCAAGGTG	TTCGAGGGGGAAATGAGAGA	55.0	50.0	58.3	57.8	354-372 bp	1.0	84.6
rps2-rpoC2_p4	GCTTCCAGCATCTCTTCCAA	TCGAGGGGGAAATGAGAGAT	50.0	50.0	57.9	57.5	357-375 bp	1.0	84.0
rps2-rpoC2_p5	TCCAGCATCTCTTCCAAGGT	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.3	57.8	355-373 bp	1.0	83.9