Hotspot

trnS-GCU-trnG-UCC

LSC · 8459-9230 · 772 bp

Back to Hemiboea

Pi 0.0215

MarkerSeek score 65.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 57
pi: 0.0215
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	833-855 bp	1.0	61.2
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	832-854 bp	1.0	61.2
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	835-857 bp	1.0	60.2
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	834-856 bp	1.0	60.2
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	887-909 bp	1.0	59.7