Hotspot

rps16-trnQ-UUG

LSC · 6076-7139 · 1064 bp

Back to Hemiboea

Pi 0.0129

MarkerSeek score 68.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1064
variable_sites: 65
pi: 0.0129
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	TCGGAGGTTCGAATCCTTTC	50.0	50.0	57.9	57.3	1136-1160 bp	1.0	81.9
rps16-trnQ-UUG_p2	AACGGATCGTGTCCTTCAAG	TAAGGCAACGGGTTTTGGTC	50.0	50.0	57.9	58.7	1163-1187 bp	1.0	81.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TCGGAGGTTCGAATCCTTTC	45.0	50.0	58.1	57.3	1119-1143 bp	1.0	81.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1146-1170 bp	1.0	81.3
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	TCGGAGGTTCGAATCCTTTC	50.0	50.0	58.2	57.3	1137-1161 bp	1.0	80.9