Hotspot

atpF

LSC · 12796-14438 · 1643 bp

Back to Hemerocallis

Pi 0.000812

MarkerSeek score 59.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1643
variable_sites: 2
pi: 0.000812
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCGAAGAGTTGCCATTAGTGT	AGGGCGAGCCAAGTGATATA	42.9	50.0	57.3	58.3	1809-1810 bp	1.0	64.4
atpF_p2	TCGAAGAGTTGCCATTAGTGT	GGGCGAGCCAAGTGATATAA	42.9	50.0	57.3	57.5	1808-1809 bp	1.0	62.4
atpF_p3	CGAAGAGTTGCCATTAGTGTC	AGGGCGAGCCAAGTGATATA	47.6	50.0	57.0	58.3	1808-1809 bp	1.0	61.9
atpF_p4	CGAAGAGTTGCCATTAGTGT	AGGGCGAGCCAAGTGATATA	45.0	50.0	55.8	58.3	1808-1809 bp	1.0	60.1
atpF_p5	CGAAGAGTTGCCATTAGTGTC	GGGCGAGCCAAGTGATATAA	47.6	50.0	57.0	57.5	1807-1808 bp	1.0	59.8