Hotspot

petL-petG

LSC · 66396-66589 · 194 bp

Back to Hemerocallis

Pi 0.00344

MarkerSeek score 35.90

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 1
pi: 0.00344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TCGGTTTTCTATTGGCTGCT	CCCCGCGTCTGTATTGTAAA	45.0	50.0	57.5	58.0	365 bp	1.0	76.2
petL-petG_p2	TCGGTTTTCTATTGGCTGCT	CAAAGGTCCAACTGATCCCC	45.0	55.0	57.5	58.2	381 bp	1.0	75.0
petL-petG_p3	TCGGTTTTCTATTGGCTGCT	ATCCCCGCGTCTGTATTGTA	45.0	50.0	57.5	58.6	367 bp	1.0	71.5
petL-petG_p4	CGGTTTTCTATTGGCTGCTTC	CCCCGCGTCTGTATTGTAAA	47.6	50.0	57.9	58.0	364 bp	1.0	71.0
petL-petG_p5	TCGGTTTTCTATTGGCTGCTT	CCCCGCGTCTGTATTGTAAA	42.9	50.0	58.2	58.0	365 bp	1.0	70.4