Hotspot

psbE-petL

LSC · 65189-66299 · 1111 bp

Back to Hemerocallis

Pi 0.0018

MarkerSeek score 55.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 3
pi: 0.0018
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1255-1257 bp	1.0	76.0
psbE-petL_p2	ACTCACGAATAACCAACCCG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1268-1270 bp	1.0	75.7
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCAGCCAATAGAAAACCGA	45.0	45.0	58.2	57.5	1264-1266 bp	1.0	75.0
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.8	57.5	1263-1265 bp	1.0	74.9
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	50.0	45.0	58.2	57.5	1257-1259 bp	1.0	74.5