Hotspot

petA-psbJ

LSC · 63583-64419 · 837 bp

Back to Hemerocallis

Pi 0.000796

MarkerSeek score 46.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 1
pi: 0.000796
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCCAAGGCCTCTTGTTCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.7	58.3	978 bp	1.0	75.4
petA-psbJ_p2	GTCCAAGGCCTCTTGTTCTT	CATATTCCGGGTTGGGTTCA	50.0	50.0	57.7	57.6	974 bp	1.0	74.4
petA-psbJ_p3	GTCCAAGGCCTCTTGTTCTT	GTTCATATTCCGGGTTGGGT	50.0	50.0	57.7	57.6	977 bp	1.0	74.4
petA-psbJ_p4	TCCAAGGCCTCTTGTTCTTC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.4	58.3	977 bp	1.0	73.0
petA-psbJ_p5	CCAAGGCCTCTTGTTCTTCT	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.4	58.3	976 bp	1.0	73.0