Hotspot

psaA-ycf3

LSC · 41962-42806 · 845 bp

Back to Hemerocallis

Pi 0.00159

MarkerSeek score 48.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 2
pi: 0.00159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTTCTTGAAAAATGGCCGGG	GGAGGCTTGGTCTGATCAAG	50.0	55.0	57.9	58.0	1054-1061 bp	1.0	79.8
psaA-ycf3_p2	GTTCTTGAAAAATGGCCGGG	CTTGGTCTGATCAAGCTGCT	50.0	50.0	57.9	57.9	1049-1056 bp	1.0	79.2
psaA-ycf3_p3	GTTCTTGAAAAATGGCCGGG	TTGGTCTGATCAAGCTGCTG	50.0	50.0	57.9	58.2	1048-1055 bp	1.0	78.6
psaA-ycf3_p4	GTTCTTGAAAAATGGCCGGG	ATAGCGCTTACCCCAGGTAA	50.0	50.0	57.9	58.2	1011-1018 bp	1.0	78.5
psaA-ycf3_p5	GTTCTTGAAAAATGGCCGGG	TAGCGCTTACCCCAGGTAAT	50.0	50.0	57.9	58.2	1010-1017 bp	1.0	78.5