Hotspot

rps14-psaB

LSC · 37335-37478 · 144 bp

Back to Hemerocallis

Pi 0

MarkerSeek score 53.90

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TCTGTTCCAATTTTTGCCGC	CCAGTGGCTCTTTCCATTGT	45.0	50.0	57.9	58.1	314-316 bp	1.0	78.6
rps14-psaB_p2	CTGTTCCAATTTTTGCCGCT	CCAGTGGCTCTTTCCATTGT	45.0	50.0	57.8	58.1	313-315 bp	1.0	78.6
rps14-psaB_p3	TCTGTTCCAATTTTTGCCGC	GCAGCTTTCTTGATTGCCTC	45.0	50.0	57.9	57.7	239-241 bp	1.0	77.3
rps14-psaB_p4	CTGTTCCAATTTTTGCCGCT	GCAGCTTTCTTGATTGCCTC	45.0	50.0	57.8	57.7	238-240 bp	1.0	77.2
rps14-psaB_p5	TCTGTTCCAATTTTTGCCGC	TGCAGCTTTCTTGATTGCCT	45.0	45.0	57.9	58.4	240-242 bp	1.0	76.5