Hotspot

psbM-trnD-GUC

LSC · 30231-31177 · 947 bp

Back to Hemerocallis

Pi 0.00141

MarkerSeek score 57.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 947
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGGCTGACTGTTTTTACGT	AGTTCAATCGGTCAGAGCAC	40.0	50.0	55.8	57.9	1109 bp	1.0	62.0
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1060 bp	1.0	61.6
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTTACGT	TGCTATACACCTCGTTGGGA	40.0	50.0	55.8	58.1	1133 bp	1.0	61.5
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	TGCTATACACCTCGTTGGGA	45.0	50.0	55.8	58.1	1084 bp	1.0	61.2
psbM-trnD-GUC_p5	TGGCTGACTGTTTTTACGTAGA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.7	57.9	1108 bp	1.0	60.9