Hotspot

psbK-psbI

LSC · 8267-8715 · 449 bp

Back to Hemerocallis

Pi 0.00148

MarkerSeek score 42.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 1
pi: 0.00148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGGCCGGGATCATTAGATA	50.0	50.0	57.9	58.1	569 bp	1.0	79.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGGCCGGGATCATTAGAT	50.0	50.0	57.9	58.1	570 bp	1.0	79.2
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTACGGCCGGGATCATTAGA	50.0	50.0	57.9	58.3	571 bp	1.0	77.3
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACGGCCGGGATCATTAGATA	45.0	50.0	58.3	58.1	566 bp	1.0	77.1
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TACGGCCGGGATCATTAGAT	45.0	50.0	58.3	58.1	567 bp	1.0	77.1