Hotspot

rps16-trnQ-UUG

LSC · 5924-7675 · 1752 bp

Back to Hemerocallis

Pi 0.000382
MarkerSeek score 59.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1752
variable_sites
1
pi
0.000382
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AATCCACAGCGAGTCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1837-1849 bp 1.0 79.8
rps16-trnQ-UUG_p2 GAATCCACAGCGAGTCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1838-1850 bp 1.0 79.8
rps16-trnQ-UUG_p3 CAGCGAGTCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1831-1843 bp 1.0 79.3
rps16-trnQ-UUG_p4 AGTCATGTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1826-1838 bp 1.0 79.3
rps16-trnQ-UUG_p5 GCGAGTCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1829-1841 bp 1.0 79.3