Hotspot

psbE

LSC · 67556-67807 · 252 bp

Back to Helwingia

Pi 0

MarkerSeek score 23.80

Primer available yes

Label psbE

Feature Metadata

label_name: psbE
feature_type: gene
region: LSC
length_bp: 252
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE_p1	ATCTATGGTCATTGGGGCCT	ATCTTTCGCGGACTCAATCC	50.0	50.0	58.2	58.1	327 bp	1.0	78.2
psbE_p2	CGATCTATGGTCATTGGGGC	ATCTTTCGCGGACTCAATCC	55.0	50.0	58.2	58.1	329 bp	1.0	78.0
psbE_p3	GATCTATGGTCATTGGGGCC	ATCTTTCGCGGACTCAATCC	55.0	50.0	57.8	58.1	328 bp	1.0	77.9
psbE_p4	ATCTATGGTCATTGGGGCCT	GGACTCAATCCCCCTTTGAC	50.0	55.0	58.2	57.9	318 bp	1.0	77.7
psbE_p5	CGATCTATGGTCATTGGGGC	GGACTCAATCCCCCTTTGAC	55.0	55.0	58.2	57.9	320 bp	1.0	77.5