Hotspot

ndhE

SSC · 121793-122098 · 306 bp

Back to Helwingia

Pi 0

MarkerSeek score 19.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ATCCTACCTTACGCGACAGA	GTTGGGGATGGGGTTACTTC	50.0	55.0	58.0	57.9	853 bp	1.0	78.7
ndhE_p2	GACCCATGCTACGAGTTGTT	GTTGGGGATGGGGTTACTTC	50.0	55.0	57.9	57.9	1274 bp	1.0	78.4
ndhE_p3	ACATCCGTCCCAAGGTATCA	GTTGGGGATGGGGTTACTTC	50.0	55.0	58.1	57.9	1141 bp	1.0	78.2
ndhE_p4	ACTTCTGGATTGGGATTGGC	GTTGGGGATGGGGTTACTTC	50.0	55.0	57.9	57.9	923 bp	1.0	77.9
ndhE_p5	ACCCGCAGATATTGGCAAAA	GTTGGGGATGGGGTTACTTC	45.0	55.0	58.2	57.9	1469 bp	1.0	77.9