Hotspot

ndhD

SSC · 119648-121150 · 1503 bp

Back to Helwingia

Pi 0.000887

MarkerSeek score 59.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 2
pi: 0.000887
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGTAAGCGAAGAAAGAGCCT	TACCGATAAAACCCGTGCTC	50.0	50.0	58.0	57.7	1691 bp	1.0	77.7
ndhD_p2	CGTAAGCGAAGAAAGAGCCT	ACGGGTTTTTCTGGTCCAAG	50.0	50.0	58.0	58.3	1640 bp	1.0	77.6
ndhD_p3	CGTAAGCGAAGAAAGAGCCT	CGGGTTTTTCTGGTCCAAGT	50.0	50.0	58.0	58.3	1639 bp	1.0	77.6
ndhD_p4	CGTAAGCGAAGAAAGAGCCT	TTTCGAGCACGGGTTTTTCT	50.0	45.0	58.0	58.3	1648 bp	1.0	77.4
ndhD_p5	CGTAAGCGAAGAAAGAGCCT	TTTTCGAGCACGGGTTTTTC	50.0	45.0	58.0	57.5	1649 bp	1.0	76.3