Hotspot

trnG-GCC-trnM-CAU

LSC · 38760-38941 · 182 bp

Back to Helwingia

Pi 0

MarkerSeek score 53.60

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 182
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	323-325 bp	1.0	79.5
trnG-GCC-trnM-CAU_p2	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	287-289 bp	1.0	79.5
trnG-GCC-trnM-CAU_p3	ATGCGGATATGGTCGAATGG	TCAGTTCTTCTCTTCAGCGC	50.0	50.0	57.9	57.9	344-346 bp	1.0	79.1
trnG-GCC-trnM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.8	58.0	294-296 bp	1.0	79.0
trnG-GCC-trnM-CAU_p5	ATGCGGATATGGTCGAATGG	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	281-283 bp	1.0	78.9