Hotspot

psbK-psbI

LSC · 8325-8719 · 395 bp

Back to Helwingia

Pi 0.00338

MarkerSeek score 48.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 2
pi: 0.00338
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	CGTCCTGGATCATTAGAGAGG	50.0	52.4	57.9	57.1	514-516 bp	1.0	64.4
psbK-psbI_p2	CGTCGATGTTATGCCAGTCA	CGTCCTGGATCATTAGAGAGG	50.0	52.4	58.1	57.1	563-565 bp	1.0	64.2
psbK-psbI_p3	TCGTCGATGTTATGCCAGTC	CGTCCTGGATCATTAGAGAGG	50.0	52.4	57.8	57.1	564-566 bp	1.0	63.2
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	CGTCCTGGATCATTAGAGAGG	45.0	52.4	58.3	57.1	511-513 bp	1.0	62.4
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	CGTCCTGGATCATTAGAGAGG	45.0	52.4	58.3	57.1	512-514 bp	1.0	62.4