Hotspot

rps16-trnQ-UUG

LSC · 5505-7736 · 2232 bp

Back to Helwingia

Pi 0.000597

MarkerSeek score 57.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 2232
variable_sites: 2
pi: 0.000597
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TTCCTTCTCTTCGGGATCGA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	2307-2308 bp	1.0	78.4
rps16-trnQ-UUG_p2	GCAACGATTTGATAGACGGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	2278-2279 bp	1.0	76.6
rps16-trnQ-UUG_p3	CGGGATCGAACATCAATTGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	2296-2297 bp	1.0	76.4
rps16-trnQ-UUG_p4	CTTCCTTCTCTTCGGGATCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	57.5	58.1	2308-2309 bp	1.0	75.5
rps16-trnQ-UUG_p5	TTCCTTCTCTTCGGGATCGA	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.9	57.3	2311-2312 bp	1.0	73.7