Hotspot

ycf1

SSC · 135772-141306 · 5535 bp

Back to Hellenia

Pi 0.00145

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5535
variable_sites: 8
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTAGGTACAGATTCAAAAGAGA	AGATTCCACAGAAATCTTTTGGA	34.8	34.8	54.5	56.1	172 bp	1.0	40.9
ycf1_p2	AACTAGGTACAGATTCAAAAGAGA	AGATTCCACAGAAATCTTTTGGA	33.3	34.8	55.2	56.1	173 bp	1.0	40.9
ycf1_p3	ACTAGGTACAGATTCAAAAGAGAA	AGATTCCACAGAAATCTTTTGGA	33.3	34.8	55.2	56.1	172 bp	1.0	40.9
ycf1_p4	TCGAAGACATATTTCAAAGATTTTGC	AGATTCCACAGAAATCTTTTGGA	30.8	34.8	57.3	56.1	264 bp	1.0	40.6
ycf1_p5	CGAAGACATATTTCAAAGATTTTGCT	AGATTCCACAGAAATCTTTTGGA	30.8	34.8	57.3	56.1	263 bp	1.0	40.6