Hotspot

rpl16

LSC · 89120-91354 · 2235 bp

Back to Hellenia

Pi 0.00378

MarkerSeek score 64.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 2235
variable_sites: 8
pi: 0.00378
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GCGGTTGTTTTTCATCCTCG	CGATTGACCATTTGTTAGAATTGC	50.0	37.5	58.0	57.4	2202-2287 bp	1.0	47.5
rpl16_p2	GCGGTTGTTTTTCATCCTCG	TGACCATTTGTTAGAATTGCT	50.0	33.3	58.0	53.7	2198-2283 bp	1.0	44.3
rpl16_p3	GCGGTTGTTTTTCATCCTCG	TTGACCATTTGTTAGAATTGCT	50.0	31.8	58.0	54.5	2199-2284 bp	1.0	44.3
rpl16_p4	TGCGGTTGTTTTTCATCCTC	CGATTGACCATTTGTTAGAATTGC	45.0	37.5	56.9	57.4	2203-2288 bp	1.0	44.3
rpl16_p5	GCGGTTGTTTTTCATCCTCG	CGATTGACCATTTGTTAGAATTGCT	50.0	36.0	58.0	58.7	2202-2287 bp	1.0	44.3