Hotspot

petA-psbJ

LSC · 69965-70988 · 1024 bp

Back to Hellenia

Pi 0.00295

MarkerSeek score 61.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1024
variable_sites: 3
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGGTCCAAGGTCTTTTGTT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.3	58.0	1153-1159 bp	1.0	77.8
petA-psbJ_p2	CGGGTCCAAGGTCTTTTGTT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.3	58.0	1151-1157 bp	1.0	77.8
petA-psbJ_p3	CGGGTCCAAGGTCTTTTGTT	TGGTATCCCTGTGATCGGTT	50.0	50.0	58.3	58.1	1202-1208 bp	1.0	77.1
petA-psbJ_p4	TACGGGTCCAAGGTCTTTTG	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.4	58.0	1155-1161 bp	1.0	75.8
petA-psbJ_p5	TACGGGTCCAAGGTCTTTTG	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.4	58.0	1153-1159 bp	1.0	75.8