Hotspot

accD-psaI

LSC · 65472-66408 · 937 bp

Back to Hellenia

Pi 0.00221

MarkerSeek score 63.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 2
pi: 0.00221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CACGGTTTCCTTCCCTTGAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1027-1037 bp	1.0	82.1
accD-psaI_p2	CACGGTTTCCTTCCCTTGAA	AGAAACCATTGCAATTGCCG	50.0	45.0	58.0	57.9	1032-1042 bp	1.0	81.6
accD-psaI_p3	AGGTCTTCTGGGCGAGTTAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1055-1065 bp	1.0	81.1
accD-psaI_p4	TCGACCCAATAGTACCACGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1086-1096 bp	1.0	80.8
accD-psaI_p5	AGGTCTTCTGGGCGAGTTAT	AGAAACCATTGCAATTGCCG	50.0	45.0	58.1	57.9	1060-1070 bp	1.0	80.7