Hotspot

petN-psbM

LSC · 32601-33993 · 1393 bp

Back to Hellenia

Pi 0.00511

MarkerSeek score 63.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1393
variable_sites: 7
pi: 0.00511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1488-1496 bp	1.0	69.5
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1489-1497 bp	1.0	69.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1547-1555 bp	1.0	69.1
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.8	57.6	1487-1495 bp	1.0	69.0
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	TGCTACTGCACTGTTCATTCT	60.0	42.9	58.2	57.6	1484-1492 bp	1.0	68.7