Hotspot

matK-trnK-UUU

LSC · 3495-4319 · 825 bp

Back to Hellenia

Pi 0.0097

MarkerSeek score 64.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 8
pi: 0.0097
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAAAGTGTTGTTGCCGAGA	GTAGGGTCGTGCAGTGTAAG	45.0	55.0	57.7	58.0	996-997 bp	1.0	78.0
matK-trnK-UUU_p2	AGAAAGTGTTGTTGCCGAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.7	58.2	905-906 bp	1.0	76.8
matK-trnK-UUU_p3	AGAAAGTGTTGTTGCCGAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.7	58.2	904-905 bp	1.0	76.8
matK-trnK-UUU_p4	AAGTGTTGTTGCCGAGATCT	GTAGGGTCGTGCAGTGTAAG	45.0	55.0	57.4	58.0	993-994 bp	1.0	76.2
matK-trnK-UUU_p5	AGAAAGTGTTGTTGCCGAGA	TAGGGTCGTGCAGTGTAAGT	45.0	50.0	57.7	58.4	995-996 bp	1.0	75.0