Hotspot

trnR-ACG-trnN-GUU

SSC · 83683-84280 · 598 bp

Back to Helleborus

Pi 0.0506

MarkerSeek score 75.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: SSC
length_bp: 598
variable_sites: 71
pi: 0.0506
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CCTTGTTCCTACATGACCCG	CAGAAATCCGTCGGCTGTTA	55.0	50.0	58.0	58.0	1327 bp	0.142857	45.5
trnR-ACG-trnN-GUU_p2	CAATTCGATCAGGCATTCGC	CAGAAATCCGTCGGCTGTTA	50.0	50.0	58.0	58.0	1305 bp	0.142857	45.5
trnR-ACG-trnN-GUU_p3	CCTTGTTCCTACATGACCCG	ATGTGGTCAGAAATCCGTCG	55.0	50.0	58.0	58.0	1334 bp	0.142857	45.4
trnR-ACG-trnN-GUU_p4	CAATTCGATCAGGCATTCGC	ATGTGGTCAGAAATCCGTCG	50.0	50.0	58.0	58.0	1312 bp	0.142857	45.4
trnR-ACG-trnN-GUU_p5	TGAAATGGCTGAGGAGAGGA	CAGAAATCCGTCGGCTGTTA	50.0	50.0	58.0	58.0	800 bp	0.142857	45.3