Hotspot

trnI-CAU-ycf2

IRb · 67336-69744 · 2409 bp

Back to Helleborus

Pi 0.078

MarkerSeek score 80.00

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 2409
variable_sites: 457
pi: 0.078
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	TCGTTCCAAGCTCGAAGTAC	50.0	50.0	58.0	57.7	2472 bp	0.142857	42.9
trnI-CAU-ycf2_p2	GTTGGGCGCTTTAACCATTC	GTTCCAAGCTCGAAGTACCA	50.0	50.0	58.0	57.5	2470 bp	0.142857	42.0
trnI-CAU-ycf2_p3	GTTGGGCGCTTTAACCATTC	CTTCATGCTCGTTCCAAGCT	50.0	50.0	58.0	58.6	2480 bp	0.142857	41.2
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	TCTTCATGCTCGTTCCAAGC	50.0	50.0	58.0	58.6	2481 bp	0.142857	41.2
trnI-CAU-ycf2_p5	GTTGGGCGCTTTAACCATTC	TTCATGCTCGTTCCAAGCTC	50.0	50.0	58.0	58.6	2479 bp	0.142857	41.2