Hotspot

petA-psbJ

LSC · 46833-47227 · 395 bp

Back to Helleborus

Pi 0.0406

MarkerSeek score 83.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 36
pi: 0.0406
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCGTGTCCAAGGTCTTTTT	TGGCTTATAGGCACTGTAGC	50.0	50.0	58.3	57.1	518-601 bp	1.0	79.2
petA-psbJ_p2	CCCGTGTCCAAGGTCTTTTT	AGGCACTGTAGCTGGTATTC	50.0	50.0	58.3	57.0	510-593 bp	1.0	78.6
petA-psbJ_p3	CCCGTGTCCAAGGTCTTTTT	GGCACTGTAGCTGGTATTCT	50.0	50.0	58.3	57.0	509-592 bp	1.0	78.6
petA-psbJ_p4	CCCGTGTCCAAGGTCTTTTT	ACTCTGGATTGGGTTCATCC	50.0	50.0	58.3	56.9	451-534 bp	1.0	78.3
petA-psbJ_p5	CCCGTGTCCAAGGTCTTTTT	GCTGGTATTCTTGTGATCGGT	50.0	47.6	58.3	58.1	500-583 bp	1.0	77.9