Hotspot

trnC-GCA-petN

LSC · 18998-19921 · 924 bp

Back to Helleborus

Pi 0.0271

MarkerSeek score 74.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 65
pi: 0.0271
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	878-987 bp	0.857143	83.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	872-981 bp	0.857143	81.7
trnC-GCA-petN_p3	TTTATCGTTTTGGCGGCATG	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.7	58.0	931-1040 bp	0.857143	81.3
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	896-1005 bp	0.857143	80.8
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	932-1041 bp	0.857143	80.1