Hotspot

trnR-UCU-atpA

LSC · 8422-8580 · 159 bp

Back to Helleborus

Pi 0.0539
MarkerSeek score 79.50
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
159
variable_sites
25
pi
0.0539
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 TTGTTTCAGCTCGGTGGAAA GCCCAATTCGCAGAGTTAGA 45.0 50.0 58.0 58.0 890-1125 bp 1.0 87.1
trnR-UCU-atpA_p2 TTGTTTCAGCTCGGTGGAAA TCTCCAGAGTAGGATCTGCG 45.0 55.0 58.0 58.0 961-1196 bp 1.0 87.1
trnR-UCU-atpA_p3 TTGTTTCAGCTCGGTGGAAA CGGTGGAAGAACAGGTAGTG 45.0 55.0 58.0 57.9 763-998 bp 1.0 87.0
trnR-UCU-atpA_p4 TTGTTTCAGCTCGGTGGAAA GGTCGTGAAGCTTATCCAGG 45.0 55.0 58.0 58.1 1174-1440 bp 1.0 86.3
trnR-UCU-atpA_p5 TTGTTTCAGCTCGGTGGAAA AATTCTGGAATCCGACCTGC 45.0 50.0 58.0 58.0 1198-1236 bp 0.857143 76.2